A reliable method for organ culture of neonatal mouse retina with long-term survival.

Study of Tnp1, Tekt1, and Plzf Genes Expression During an in vitro Three-Dimensional Neonatal Male Mice Testis Culture

Background: In vitro spermatogenesis has a long research history beginning in the early 20th century. This organ culture method was therefore abandoned, and alternative cell culture methods were chosen by many researchers. Here, whether Tnp1, Tekt1, and Plzf, which play a crucial role in spermatogenesis, can be expressed during testis organ culture was assessed. Methods: Testes of 10 mouse pups...

بررسی عواقب دراز مدت و فاکتورهای پیش‌بینی کننده درگیری ارگانها در اسکلروز سیستمیک

ABSTRACT This study is done to compare the long term outcome in two group of patient withsystemic sclerosis(SSc), diffuse cutaneous type(dcSSc) versus limited type(lcSSc) and determine predictive factors for specific organ involvement. Patient had to meet American College of Rheumathology(ACR) criteria for clacification of SSC eligible to study. In this study only clinically detectable...

Reactive oxygen species level, mitochondrial transcription factor A gene expression and succinate dehydrogenase activity in metaphase II oocytes derived from in vitro cultured vitrified mouse ovaries

The aim of this study was to evaluate the effects of ovarian tissue vitrification and two-step in vitro culture on the metaphase II (MII) oocyte reactive oxygen species (ROS) level, mitochondrial transcription factor A (TFAM) expression and succinate dehydrogenase (SDH) activity. After collection of neonatal mouse ovaries, 45 ovaries were vitrified and the others (n = 45) were...

Steroid Production and Follicular Development of Neonatal Mouse Ovary during In Vitro Culture

Mouse retina explants after long-term culture in serum free medium.

The neonatal mouse retina remains viable as an explant in serum-supplemented growth media for more than 4 weeks. Interpretation of drug effects on this tissue is compromised by the enigmatic composition of the serum. We sought to remove this ambiguity by culturing neonatal as well as late postnatal mouse retina in serum-free nutrient medium. In this study three important observations were made,...